AI-Enhanced 3D Bioprinting

Abstract

Bioprinting fabricates living tissues by layering cells, bioinks, and biomaterials through 3D printing techniques [1]3D bioprinting builds life-like tissue structures by depositing bioink blends containing living cells [2]AI-driven bioprinters arrange living cells with precision to mimic the intricate design of human tissues [3].Miniature organoids could revolutionize drug testing, disease models, and, in the future, organ transplantation [3].

Introduction

AI significantly enhances 3D bioprinting by automating processes, reducing human error, and ensuring consistent tissue production. It enables real-time adjustments during printing, optimizing bioink formulations, medical imaging, and printing parameters to create precise tissue structures such as skin grafts, improving transplantation outcomes.

The field of bioprinting emerged alongside advancements like the full mapping of the human genome and milestones such as the creation of urinary bladders from patient cells by Harvard researchers. AI-driven quality control is transforming tissue engineering by simplifying parameter optimization, improving regulatory compliance, and enabling personalized regenerative medicine.

Methodologically, stem cells are favored for bioprinting due to their proliferation capabilities and functional similarity to original cells. Bioinks and hydrogels—materials that mimic the extracellular matrix—are critical for supporting cell growth and tissue regeneration. AI manages large datasets and complex calculations throughout the bioprinting workflow, from imaging to printing, reducing waste and improving material selection.

The study includes AI-assisted scaffold design simulated in MATLAB, where scaffold structures are optimized using random factors to mimic AI adjustments. Tools like PrusaSlicer and Google Colab facilitate real-time feedback and data visualization, highlighting AI’s role in optimizing 3D bioprinting processes.

Conclusion

AI enhances bioprinting automation, minimizing human error and ensuring consistent tissue production [4].AI-driven automated bioprinting adjusts in real-time to perfect tissue structures like skin grafts, enhancing patient outcomes in transplantation by optimizing bioink, medical imaging, and printing techniques [5].AI in bioprinting automates medical imaging, predicts ideal bioink compositions, and adjusts printing parameters in real time to ensure precision and compatibility[6].Thomas Boland pioneers the first bioprinter as the human genome is fully mapped, both physically and functionally [7].3D bioprinting entered medicine when Harvard researchers at Boston Children\'s Hospital created the first hand-built urinary bladders by layering patient cells onto collagen and polymer scaffolds [8].

References

[1] Cellink. (n.d.). Bioprinting explained simply. Retrieved from https://www.cellink.com/blog/bioprinting-explained-simply/ [2] UPM Biomedicals. (n.d.). What is 3D bioprinting?. Retrieved from https://www.upmbiomedicals.com/solutions/life-science/what-is-3d-bioprinting/#:~:text=3D%20bioprinting%20is%20a%20technology,engineering%20and%20new%20drug%20development [3] Santana, J. A. (2023). Printing the future: AI-powered bioprinters weave tissue for medical miracles. Medium. Retrieved from https://medium.com/@jamesasantana/printing-the-future-ai-powered-bioprinters-weave-tissue-for-medical-miracles-7e9cb0a871fc#:~:text=Bioprinters%2C%20guided%20by%20AI%20algorithms%2C%20meticulously%20layer,testing%2C%20disease%20modeling%2C%20and%20ultimately%2C%20organ%20transplantation [4] Financial Express. (2023, March 19). Revolutionizing organ transplants: How AI is making bioprinting of human tissues a reality. Retrieved from https://www.financialexpress.com/opinion/revolutionizing-organ-transplants-how-ai-is-making-bioprinting-of-human-tissues-a-reality/3657532/ [5] AIP Publishing. (2023). Recent advances and applications of artificial intelligence in bioprinting. Bioprinting, 5(3), 031301. https://pubs.aip.org/aip/bpr/article-abstract/5/3/031301/3303852/Recent-advances-and-applications-of-artificial?redirectedFrom=fulltext [6] AIP Scilights. (2023, February 20). Employing artificial intelligence to augment 3D bioprinting. Retrieved from https://ww2.aip.org/scilights/employing-artificial-intelligence-to-augment-3d-bioprinting [7] BioLife4D. (n.d.). History of bioprinting. Retrieved from https://biolife4d.com/history-of-bioprinting/#:~:text=Thomas%20Boland%20creates%20the%20first%20bioprinter.&text=Completion%20of%20computer%20mapping%20of,both%20physical%20and%20functional%20standpoints [8] Clemson University. (2021). What is 3D bioprinting? Retrieved from https://opentextbooks.clemson.edu/sts1010fidlerfall2021/chapter/3d-bioprinting/#:~:text=3D%20bioprinting%20was%20first%20introduced,patients%20to%20grow%20into%20functioning [9] IJRASET. (2023). Artificial intelligence in 3D bioprinting. International Journal for Research in Applied Science and Engineering Technology, 11(3), 2454-2459. Retrieved from https://www.ijraset.com/research-paper/artificial-intelligence-in-3d-bio-printing [10] Springer. (2023). Chapter 14: AI in 3D Bioprinting. In Emerging Technologies in Bioprinting (pp. 283-298). Springer. Retrieved from https://link.springer.com/chapter/10.1007/978-981-97-3048-3_14 [11] PubMed. (2023). AI applications in bioprinting and tissue engineering: A review. National Library of Medicine. Retrieved from https://pubmed.ncbi.nlm.nih.gov/39690752/ [12] MDPI. (2023). Recent advancements in bioinks for 3D bioprinting applications. Micromachines, 13(3), 363. Retrieved from https://www.mdpi.com/2072-666X/13/3/363 [13] AIP Publishing. (2023). Recent advances and applications of artificial intelligence in bioprinting. Bioprinting, 5(3), 031301. https://pubs.aip.org/aip/bpr/article-abstract/5/3/031301/3303852/Recent-advances-and-applications-of-artificial?redirectedFrom=fulltext [14] PubMed. (2023). Artificial intelligence in 3D bioprinting for tissue regeneration. National Library of Medicine. Retrieved from https://pubmed.ncbi.nlm.nih.gov/39036708/ [15] Sigma-Aldrich. (n.d.). 3D bioprinting bioinks. Retrieved from https://www.sigmaaldrich.com/IN/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/3d-cell-culture/3d-bioprinting-bioinks [16] Nature. (2021). Hydrogels in 3D bioprinting: Applications and potential in tissue regeneration. Nature Biomedical Engineering, 5(9), 889-904. Retrieved from https://www.nature.com/articles/s41392-021-00830-x#:~:text=Hydrogels%20are%20a%20class%20of,tissue%20regeneration%20and%20disease%20therapies [17] DesignTech Products. (2023). AI in 3D printing. Retrieved from https://www.designtechproducts.com/articles/ai-3d-printing#:~:text=AI%20can%20also%20be%20used%20to%20optimize%20the%20printing%20process,is%20left%20to%20human%20skills [18] ScienceDirect. (2024). AI and 3D bioprinting: Methodologies and future directions. Advanced Drug Delivery Reviews, 91, 1-20. Retrieved from https://www.sciencedirect.com/science/article/pii/S2452199X2400505X#:~:text=5b).,to%20quantitatively%20assess%20model%20performance.,to%20quantitatively%20assess%20model%20performance) [19] PubMed. (2023). 3D bioprinting for skin wound healing and regeneration. National Library of Medicine. Retrieved from https://pmc.ncbi.nlm.nih.gov/articles/PMC9295304/#:~:text=3D%20bioprinting%20could%20offer%20several,outlook%20of%20skin%20wound%20healing [20] ScienceDirect. (2023). Artificial intelligence applications in 3D bioprinting for tissue engineering. Journal of Biochemical Engineering, 98, 102834. Retrieved from

Copyright

Copyright © 2025 Sriganesh V. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.