Ijraset Journal For Research in Applied Science and Engineering Technology
Authors: Namrata Dhadnekar, Shamal Tavhare, Sayali Gaikwad, Keerthi Raja Rajeshwari, Fani Krishna Chenagani
DOI Link: https://doi.org/10.22214/ijraset.2026.77142
Certificate: View Certificate
Nashik, Maharashtra has seen an extensive spread of food adulteration issues, with emerging populations being especially at risk from its consequences. Milk is regarded as a complete food since it is rich in vitamins and minerals that support healthy bodily growth. According to the Department of Animal Husbandry and Dairying report 2021-22, about 878.94 tonnes of milk were produced. However, the quantity generated by producers is lower than that of consumers. Many common adulterants are used to enhance volume, simulate nutritious content, prolong shelf life and improve profit. This addition decreases the nutritive value of milk. Health risks from consuming adulterated milk include bacterial infections, chronic toxicity, cancer, gastrointestinal issues, renal damage, nutritional deficiencies and other health issues that rely on the adulterant. Thirty milk samples, comprising both packaged and dairy variants were passed through qualitative analysis to determine adulterants including formalin, salts, sugar, detergent, starch, and skimmed milk. Standard chemical tests were conducted to ascertain the presence of these substances. The results pointed out that a substantial measure of the samples contained adulterants, with ammonium salt, common salt and formalin being the contaminants detected most often. Compared to dairy milk, packaged milk samples showed elevated levels of adulteration, accompanied by traces of preservatives such as formalin detected as well. This research highlights the immediate necessity for more stringent quality control measures and regular testing to safeguard the safety of milk.
Milk plays a vital nutritional and economic role in India, where dairying has been a traditional livelihood and the country is the world’s largest milk producer. In its natural form, milk is a highly nutritious food containing proteins, fats, carbohydrates, vitamins, and minerals. However, milk quality is frequently compromised by bacterial contamination and, more seriously, by widespread adulteration driven largely by profit motives.
Milk adulteration in India is a major public health and ethical issue. Common adulterants include water, detergents, urea, starch, sugar, salts, preservatives (such as formalin and hydrogen peroxide), vegetable fats, pesticides, and even milk from other animals. These substances are added to increase volume, mask dilution, enhance density, prolong shelf life, or falsely elevate protein and fat content. Such practices can cause serious health problems, including organ damage, endocrine disruption, cancer, and metabolic disorders.
The text reviews major categories of milk adulterants, their purposes, and associated health risks, highlighting substances like detergents, ammonium sulphate, formalin, starch, cane sugar, sodium chloride, and skim milk powder. It also summarizes findings from multiple studies conducted in India and elsewhere, which show alarmingly high rates of milk adulteration and emphasize the limitations of traditional detection methods.
Various qualitative and quantitative techniques for detecting adulteration are discussed, ranging from simple chemical tests and lactometer readings to advanced analytical approaches such as mid-infrared spectroscopy, chromatography, electrophoresis, hyperspectral imaging, and machine learning–based systems. While technological advances have improved detection, gaps remain in real-world implementation, consumer awareness, and regulatory enforcement.
Overall, the text underscores that milk adulteration remains a serious challenge in India, threatening consumer health and trust. It calls for continued research, better detection technologies, stronger regulatory measures, and increased public education to ensure milk safety and quality.
One of the main motivations for milk adulteration is financial gain. The majority of the milk samples had adulterants introduced either purposefully or during manufacture and processing in accordance with one\'s own decision to make money. Because milk can be tainted with subpar, less expensive components or toxic substances including pond water, milk powder, cane sugar, urea, melamine, glucose, and detergents, tainted milk and milk products pose a threat to all of humanity. A number of health issues arise when people consume such contaminated and fake milk. High quality protein, important amino acids, and other vitamins and minerals, including as calcium, are all abundant in milk and are critical for healthy growth and development. Milk contributes to energy needs by providing fats and carbs. There are compounds in milk that help strengthen the immune system. Milk can help with vitamin absorption and digestion. However, there are serious health hazards associated with milk adulteration, which involves the addition of dangerous compounds, such as nutritional deficits, digestive issues, and even possibly toxic effects. Therefore, in order to guarantee the best possible health and wellbeing, it is essential to recognize and stay away from contaminated milk. Respondents from the low-income category were the least educated, less aware of their rights and obligations, and less aware of food adulteration. milk is a staple and complete diet in India, this analysis is done to educate the public about the health risks associated with milk production malpractices. To prevent milk adulteration in our nation, strict regulations, public education, and a well-organized dairy business are needed. Each and every departing human being must be aware of food adulteration.
[1] Chugh, R., & Kaur, G. (2022, February). A Study on Milk Adulteration and methods of detection of various Chemical Adulterants qualitatively. In IOP Conference Series: Materials Science and Engineering (Vol. 1225, No. 1, p. 012046). IOP Publishing. [2] Ahirwar, R., Harilal, P. T., Srihari, K. A., & Pandey, M. C. (2015). Quality changes in milk adulterated with detergent, urea, ammonium sulphate and neutralizers. Asian Journal of Dairy and Food Research, 34(4), 285-289. [3] Choudhary, M., & Sharma, N. (2024). Milk adulterants: Serious impact on human health. Journal of Medical Evidence, 5(2), 124-128. [4] Yadav, A. K., Gattupalli, M., Dashora, K., & Kumar, V. (2023). Key milk adulterants in India and their detection techniques: A review. Food Analytical Methods, 16(3), 499-514. [5] Reddy, D. M., Venkatesh, K., & Reddy, C. V. S. (2017). Adulteration of milk and its detection: a review. International Journal of Chemical Studies, 5(4), 613-617. [6] milk composition and its constituents, Dr Narendra K Nayak department of livestock products technology college of veterinary science & A.H., Mhow [7] Chauhan, S. L., Priyanka, K. D. M., Paul, B. R., & Maji, C. (2019). Adulteration of milk: A review. IJCS, 7(1), 2055-2057. [8] Afzal, A., Mahmood, M. S., Hussain, I., & Akhtar, M. (2011). Adulteration and microbiological quality of milk (a review). Pakistan Journal of Nutrition, 10(12), 1195-1202. [9] Unit-8 Milk Composition, its Constituents and Nutritional Importance. URL: http://hdl.handle.net/123456789/9289. [10] Patil, G. B., Wani, S. P., Bafna, P. S., Bagul, V. S., Kalaskar, M. G., & Mutha, R. E. (2024). Milk adulteration: From detection to health impact. Food and Humanity, 100339. [11] Das, S., Goswami, B., & Biswas, K. (2016). Milk adulteration and detection: a review. Sensor letters, 14(1), 4-18. [12] Kapoor, R., Chitranshi, R., Mishra, S., Mishra, Y. K., & Srivastav, S. P. (2021). Effect of Adulteration in Milk Concerning with Human Health in India. IJCRMMS, 4(2), 16-33. [13] Strucken EM, Laurenson YC, Brockmann GA. Go with the flow-biology and genetics of the lactation cycle. Front Genet. 2015 Mar 26;6:118. doi: 10.3389/fgene.2015.00118. PMID: 25859260; PMCID: PMC4374477. [14] Managing Cow Lactation Cycle [15] Mohanty, T. J., Sahoo, J. P., & Samal, K. C. Common milk adulteration in India and rapid detection techniques. Food Sci. Reports, 1(10), 59. [16] Azad, T., & Ahmed, S. (2016). Common milk adulteration and their detection techniques. International Journal of Food Contamination, 3, 1-9. [17] Poonia, A., Jha, A., Sharma, R., Singh, H. B., Rai, A. K., & Sharma, N. (2017). Detection of adulteration in milk: A review. International journal of dairy technology, 70(1), 23-42. [18] Kandpal, S. D., Srivastava, A. K., & Negi, K. S. (2012). Estimation of quality of raw milk (open & branded) by milk adulteration testing kit. Indian journal of community health, 24(3), 188-192. [19] Ceniti, C., Spina, A. A., Piras, C., Oppedisano, F., Tilocca, B., Roncada, P., ... & Morittu, V. M. (2023). Recent advances in the determination of milk adulterants and contaminants by mid-infrared spectroscopy. Foods, 12(15), 2917. [20] Nascimento, C. F., Santos, P. M., Pereira-Filho, E. R., & Rocha, F. R. (2017). Recent advances on determination of milk adulterants. Food chemistry, 221, 1232-1244. [21] Perez-Gonzalez, C., Garcia-Hernandez, C., Garcia-Cabezon, C., Rodriguez-Mendez, M. L., Dias, L., & Martin-Pedrosa, F. (2024). Analysis of milk adulteration by means of a potentiometric electronic tongue. Journal of Dairy Science, 107(11), 9135-9144. [22] Ghrissi, H., Veloso, A. C. A., Marx, Í. M. G., Dias, T., & Peres, A. M. (2021). A Potentiometric Electronic Tongue as a Discrimination Tool of Water-Food Indicator/Contamination Bacteria. Chemosensors, 9(6), 143. https://doi.org/10.3390/chemosensors9060143 [23] Aqeel, M., Sohaib, A., Iqbal, M., & Ullah, S. S. (2025). Milk adulteration identification using hyperspectral imaging and machine learning. Journal of Dairy Science, 108(2), 1301-1314. [24] Hemanth Singuluri, H. S., & Sukumaran, M. K. (2015). Milk adulteration in Hyderabad, India-a comparative study on the levels of different adulterants present in milk. [25] Kamthania, M., Saxena, J., Saxena, K., & Sharma, D. K. (2014). Milk Adultration: Methods of Detection &Remedial Measures. International Journal of Engineering and Technical Research, 1, 15-20. [26] Nagraik, R., Sharma, A., Kumar, D., Chawla, P., & Kumar, A. P. (2021). Milk adulterant detection: Conventional and biosensor based approaches: A review. Sensing and Bio-Sensing Research, 33, 100433. [27] Neto, H. A., Tavares, W. L., Ribeiro, D. C., Alves, R. C., Fonseca, L. M., & Campos, S. V. (2019). On the utilization of deep and ensemble learning to detect milk adulteration. BioData Mining, 12, 1-13. [28] Nawaz, T., Rehman, Z. U., Ullah, R., Ahmed, N., & Sayed, S. M. (2022). Physicochemical and adulteration study of fresh milk collected from different locations in Pakistan. Saudi Journal of Biological Sciences, 29(12), 103449. [29] Garg, L., & Mulla, S. (2024). Qualitative Assessment for Milk Adulteration: Extent, Common Adulterants, and Utility of Rapid Tests. Indian Journal of Community Medicine, 49(5), 747-751. [30] Kumar, A., Goyal, S. K., Pradhan, R. C., & Goyal, R. K. (2015). A study on status of milk adulterants using in milk of district Varanasi. South Asian J Food Technol Environ, 1, 140-143. [31] Gawali, S. P. (2021). Common milk adulteration and their detection techniques: A review. International Journal of Multidisciplinary Educational Research, 10(2/5). [32] Sen, S., Donthula, H., Ravindar, B., Jala, S., Mitta, C., & Kalepu, S. (2022). Qualitative And Quantitative Analysis For Adulteration And Nutritional Value Of Milk: A Case Study. Neuroquantology, 20(17), 227-237. [33] Raturi, N., Aman, J., & Sharma, C. (2022). Study of adulteration in milk and milk products and their adverse health effects. Octa Journal of Biosciences, 10(1). [34] Patari, S., Datta, P., & Mahapatra, P. S. (2022). 3d paper-based milk adulteration detection device. Scientific Reports, 12(1), 13657. [35] Chavatte?Palmer, P., Arnaud, G., Duvaux?Ponter, C., Brosse, L., Bougel, S., Daels, P., ... & Palmer, E. (2002). Quantitative and qualitative assessment of milk production after pharmaceutical induction of lactation in the mare. Journal of veterinary internal medicine, 16(4), 472-477. [36] Kumar, V., Aulakh, R. S., Gill, J. P. S., & Sharma, A. (2022). Exploring smart phone based colorimetric technology for on-site quantitative determination of adulterant (neutralizer) in milk. Journal of Food Science and Technology, 59(9), 3693-3699. [37] Shalileh, F., Sabahi, H., Dadmehr, M., & Hosseini, M. (2023). Sensing approaches toward detection of urea adulteration in milk. Microchemical Journal, 193, 108990. [38] Sharma, R., Seth, R., & Bauri, A. K. (2011). Rapid methods for detection of adulterants in milk. Chemical Analysis of Value Added Dairy Products and Their Quality Assurance, 11, 184-185. [39] Aparnathi, K. D., Shaikh, A. I., & Patel, S. I. (2020). Qualitative tests for detection of common adulterants in milk. Director of Research, Anand Agricultural University, Anand-388110. [40] Chaudhari, P. R., Kakade, P. V., Patel, S. I., Shaikh, A. I., & Aparnathi, K. D. (2018). Effect of temperature on performance of qualitative tests for detection of common adulterants and preservatives in milk. Indian J Dairy Sci, 71(5), 441-446. [41] Navale, D., & Gupta, S. (2016). To test an adulteration present in milk Sample. Int J Latest Technol Eng Manag Appl Sci, 5, 86-89. [42] Kitazume, E., Koikawa, S., Hui, L., Sannohe, S., Yang, Y., Maki, Y., & Ito, Y. (2012). Sequential determination of anionic-type detergents by complexation with methylene blue using dual high speed counter-current chromatography. Journal of Chromatography A, 1236, 148-151. [43] Besir, A., Yazici, F., Mortas, M., & Gul, O. (2021). A novel spectrophotometric method based on Seliwanoff test to determine 5-(Hydroxymethyl) furfural (HMF) in honey: Development, in house validation and application. Lwt, 139, 110602. [44] Saad, M. F., Kassem, M. G., & Abdel-Latif, F. E. (2021). New application of Hehner\'s test with modification for detection of formalin in cheeses and fish. Chemical Papers, 75, 4969-4972.
Copyright © 2026 Namrata Dhadnekar, Shamal Tavhare, Sayali Gaikwad, Keerthi Raja Rajeshwari, Fani Krishna Chenagani. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Paper Id : IJRASET77142
Publish Date : 2026-01-26
ISSN : 2321-9653
Publisher Name : IJRASET
DOI Link : Click Here
Submit Paper Online
