Formulation and Evaluation of Giloy Berberine-Loaded Hydrocolloidal Patches for Acne Treatment

Abstract

Giloy, scientifically known as Tinospora Cordifolia, is a widely recognized medicinal plant in Ayurveda. It is a deciduous climbing shrub with heart-shaped leaves belonging to Menispermaceae family, acting as one of the main sources of new pharmaceuticals and health care products. The plant is genetically diverse, containing different active components, including steroids, aliphatics, alkaloids, glycosides, and diterpenoid lactones. This herb possesses anti- inflammatory, antibacterial, immunomodulating agent, anti-viral, anti-diabetic properties in addition to being a strong antioxidant.1 A primary method for determining the antimicrobial activity of a compound and its active substance was carried out on invitro model through agar well diffusion method on Propionobacterium Acne. Observations of antibacterial activity were carried out by reviewing clear zones indicating the inhibition zones.2 Studies revealed that T. cordifolia is an excellent drug and does not have any adverse or toxic effects till now. There is a new invention for topical acne treatment called acne patch. The purpose of this study was to formulate an acne patch preparation from Giloy Stem extract with a combination of Xanthan gum and Propylene glycol and other excipients against Propionibacterium acnes bacteria. Acne patch preparation was evaluated for physical characteristics, and tested for its antibacterial effectiveness activity.

Introduction

1. Overview: Acne and Patch-Based Treatments

• Acne, especially in teenagers, remains a widespread issue.

• Scientists have developed topical acne patches as an effective treatment method.

• Most commercial patches are made from hydrocolloid or hydrogel materials, offering a protective barrier, moisture retention, and bacteria prevention.

2. Types of Acne Patches

? Hydrocolloid Patches

• Work on surface acne (whiteheads) by targeting the stratum corneum.

• Absorb pus, hydrate skin, and protect the lesion.

• Passive, non-medicated mechanism; effective for overnight healing.

3. Tinospora cordifolia (Giloy): A Herbal Ingredient

• Giloy is a tropical climbing shrub from the Indian subcontinent.

• Used in traditional medicine for various therapeutic effects.

Chemical Constituents:

4. Patch Formulation Using T. cordifolia

Materials & Chemicals Used:

• Plant extract (ethanolic), Xanthan gum, Aloe vera gel, Tea tree oil, Propylene glycol, Triethanolamine, Distilled water

Key Equipment:

Includes magnetic stirrer, UV spectrophotometer, incubator, pH meter, hot air oven, and others.

Formulation Steps:

1. Drying and grinding of T. cordifolia stem.

2. Cold maceration using ethanol to extract active compounds.

3. Preparation of patch matrix using aloe vera, xanthan gum, and plant extract.

4. Casting and drying the patch in Petri dishes for 24 hours at room temperature.

5. Evaluation and Testing

• Physical properties of extract: Light brown color, powdered form.

• Phytochemical screening confirmed the presence of alkaloids using standard reagent tests (Dragendorff’s, Mayer’s, etc.).

• Column chromatography and TLC were used for compound purification and identification.

  • TLC performed using a hexane:ethyl acetate solvent system (4.3:1.8).

Conclusion

Tinospora cordifolia holds significant potential as a natural ingredient in cosmetics due to its numerous therapeutic properties. The stem extract of T. cordifolia has shown antibacterial potential against major acne causing bacteria P.acne. The aim of the current research work is to prepare herbal acne patches by using herbal and natural ingredients. Formulation were prepared and evaluated for organoleptic properties and other evaluation parameters including pH, thickness, moisture absorption, folding resistance. Organoleptic tests show that F1 and F2 are transparent, while F3 is little bit light brown. All patch formulations have a thickness of 0.22-0.25mm, folding endurance 7-15 times, moisture absorption of 15.5%.Studies revealed that T. cordifolia is an excellent drug and does not have any adverse or toxic effects till now.

References

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Copyright

Copyright © 2025 Divya Borade, Sanskruti Bambale, Prajwali Bhalerao. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.