Screening of Brevundimonas Terrae for Production of Amylase

Abstract

Brevundimonas terrae is a lesser-known but ecologically significant bacterium found in various terrestrial environments. This study explores its morphological, physiological, and biochemical characteristics. By analyzing its growth conditions and potential applications, we aim to contribute to the understanding of this microorganism\'s role in environmental and industrial settings. Additionally, we performed 16S rRNA sequencing, BLAST analysis, and phylogenetic tree construction to determine its taxonomic position and evolutionary relationships.

Introduction

Brevundimonas terrae is a Gram-negative, aerobic, motile bacterium belonging to the Alphaproteobacteria class and Caulobacteraceae family. Commonly found in soil, water, and extreme environments, it displays strong adaptability and can utilize multiple carbon sources such as glucose, sucrose, and maltose. Because of its pollutant-degrading abilities and interactions with plants, it holds potential for bioremediation and agricultural applications. However, research on its genetics and functional capabilities remains limited.

The study aimed to investigate the morphological and biochemical characteristics of B. terrae, determine its optimal growth conditions, and perform molecular identification through 16S rRNA sequencing, BLAST comparison, and phylogenetic analysis.

Methods

Soil samples were collected from garden soil and the bacterium was isolated using serial dilution and plating on nutrient agar. Morphological studies, Gram staining, and biochemical tests (oxidase, catalase, starch hydrolysis, carbohydrate utilization) were conducted. Growth was assessed under varying temperature and pH conditions. The 16S rRNA gene was amplified via PCR, sequenced, analyzed through BLAST, and used to construct a phylogenetic tree in MEGA software.

Results and Discussion

B. terrae formed small yellow colonies and showed characteristics typical of the genus: Gram-negative rods with a polar flagellum, oxidative metabolism, and broad substrate utilization. Optimal growth occurred at 28–30°C and pH 7, confirming its environmental versatility.

16S rRNA sequencing verified its identity, showing high similarity to species within the Brevundimonas genus and placing it phylogenetically close to B. subvibrioides. These molecular findings reinforce its taxonomic placement.

Due to its metabolic flexibility and ability to degrade hydrocarbons, B. terrae shows promise for environmental and industrial applications, particularly in bioremediation. Further genomic and functional studies are recommended to fully explore its potential.

Conclusion

This study provides a foundational understanding of Brevundimonas terrae, emphasizing its adaptability and potential industrial applications. The inclusion of 16S rRNA sequencing, BLAST analysis, and phylogenetic studies enhances the accuracy of its classification. Future research should focus on its genetic characterization and practical applications in biotechnology, industry and environmental management.

References

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Copyright

Copyright © 2025 Dr. Gare S. S., Swami S. S., Pawar S. J.. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.