Some novel series of pyrazoline derivatives were synthesized from Chalcones. Various Pyrazoline derivatives were prepared by reflux reaction of Chalcone with Phenyl Hydrazine Hydrate in ethanolic solution. The structures of the newly synthesized Pyrazoline derivatives have been characterized by spectral data.
Because bacterial resistance to antibacterial drugs is rapidly developing, it is critical to develop new scaffolds for the design and synthesis of new antibacterial agents to help in the fight against harmful germs. Chalcones are a class of natural and synthetic compounds that have a wide variety of pharmacological actions, including antibacterial1, antitumour2, anticancer3, antitubercular4, antiinflammatory5, antioxidant6, antimalarial7, antileishmanial8, and other properties. The biological activity of chalcones is determined to be due to the presence of a reactive, -unsaturated keto group. In this study, chalcones were made by condensing different ketones with aromatic aldehyde via claisen Schmidt condensation. According to available evidence, N-containing heterocyclic compounds derived from chalcones have a wide range of activities9-17, including possible cytotoxic, antibacterial, antiviral, anti-inflammatory, anaesthetic, and mydriatic properties.
II. MATERIALS AND METHOD
All of the reagents and solvents used were of the highest quality. Which were used without being purified further. Melting points were measured using standardized melting point equipment. UV-Visible absorption spectra in DMSO were recorded using a SHIMADZU UV-1800 spectrometer with more than a concentration range of 104 M. In KBr pellets, IR spectra were obtained on an FT-IR-Alpha Bruker IR spectrometer from 4000 cm1 to 400 cm1. The 1H and 13C NMR spectra in DMSO-d6 were acquired at 500 MHz using an AV500 Resolution Multinuclear FT-NMR Spectrometer with tetramethylsilane as the internal standard. In sterile distilled water, a working stock of 100 mg/ml concentration of the given drug samples identified as "pyrazoline derivatives" was prepared.
(Z)-2-benzylidene-N1-(((2-hydroxy-3-nitrophenyl)(4-methoxyphenyl)methyl)carbamothioyl)-N3-(((2-hydroxyphenyl)(4-methoxyphenyl)methyl)carbamothioyl) malonamide (0.01 mole) In 100% ethanolic medium, malonamide and hydrazine hydrate (0.01 mole) were refluxed for 1 hour. When the mixture was filtered under suction and recrystallized from ethanol, it was cooled and poured on crushed ice, and recrystallized from ethanol.
In this study, we have successfully synthesized new pyrazolines derivatives compounds. The proper analysis of the synthesis of new pyrazolines derivatives has been systematically evaluated by FT-IR, NMR, Mass spectrometry.
 Hogale M.B., Dhore N.P., Shelar A.R. and Pawar P.K., Orient J. Chem., 1986, 2, 55.
 Yamakawa T., Kagechika H., Kawachi E., Hashimoto Y. and Shedo K., J. Med. Chem., 1990, 33, 1430.
 Ahluwalia V.K., Nayal L., Kalia N., Bala S. and Tahim, A.K., Indian J. Chem., 1987, 26B, 384.
 Bhatt A.K., Bhamaria R.P., Patel M.R., Bellare R.A. and Deliwala, Indian J. Chem., 1972, 10, 694.
 Mukherjee S., Kumar V., Prasad A.K., Raj H.G., Brakhe M.E., Olsen C.E., Jain S.C Bio-org. Med.Chem., 2001, 9, 337.
 Indyah S.A., Timmerman H., Samhoedi M., Sastronami., Sugiyanto and Goot H.V. Eur. J. Med. Chem., 2000, 35, 449-457.
 Chen M., Christensen S., Zhai L., Rasmussen M.H., Theander T.G., Frokjaer S., Steffensen B., Davidson J. and Kharazmi, A. J.Infect. Dis., 1997, 176(5), 1327-1333.
 Nielsen S.F., Christensen S.B., Cruciani G. Kharazmi A., and Liljefors, T., Antileishmanial chalcones, J. Med. Chem., 1998, 41, 4819-4832.
 Vibhute Y.B. and Basser M.A., Ind.J. of Chem., 2003, 42B, 202-205.
 Bhat B.A., Dhar K.L., Saxena A.K. and Shanmugavel M., Bio org. & Med. Chem., 2005, 15(3), 177-3180.
 Edwards M.L., Stemerick D.M. and Sunkara P.S., J. of Med. Chem., 1990, 33, 1948-54.
 Kalirajan R., Palanivelu M., Rajamanickam V., Vinothapooshan G. and Anandarajagopal K.,Int. J. of Chem. Sci., 2007, 5(1), 73-80.
 Udupi R. H., Bhat R., and Kumar K., Indian J. of Het.Chem., 1998, 8, 143-146.
 Pande A.and Saxena V.K., Ind.J.of Chem., 1987, 26B ,390-392.
 Gupta U., Sareen V., Khatri V. and Chugh, S., Indian J. of Het.Chem., 2005, 14, 265-266.
 Pandey V.K., Gupta V.D. and Tiwari D.N., Indian J. of Het. Chem., 2004, 13, 399-400.
 Mishra R.M. and Wahab, A., Indian J. of Het. Chem., 2003, 13, 29-32.
 Pharmacopoeia of India, 1996, II, A-100, A-108.